Age and sex differences in human skeletal muscle fibrosis markers and transforming growth factor-ß signaling

Journal article


Parker, L, Caldow, Marissa K., Watts, Rani, Levinger, P., Cameron-Smith, David and Levinger, Itamar. (2017). Age and sex differences in human skeletal muscle fibrosis markers and transforming growth factor-ß signaling. European Journal of Applied Physiology. 117(7), pp. 1463 - 1472. https://doi.org/10.1007/s00421-017-3639-4
AuthorsParker, L, Caldow, Marissa K., Watts, Rani, Levinger, P., Cameron-Smith, David and Levinger, Itamar
Abstract

Purpose The aim of the study was to determine whether higher fibrosis markers in skeletal muscle of older adults are accompanied by increased expression of components of the canonical TGF-β signal transduction pathway. Methods Fourteen healthy young (21–35 years; 9 males and 5 females) and seventeen older (55–75 years; 9 males and 8 females) participants underwent vastus lateralis biopsies to determine intramuscular mRNA and protein expression of fibrogenic markers and TGF-β signaling molecules related to TGF-β1 and myostatin. Results Expression of mRNA encoding the pro-fibrotic factors; axin 2, collagen III, β-catenin and fibronectin, were all significantly higher (all p < 0.05) in the older participants (350, 170, 298, and 641%, respectively). Furthermore, axin 2 and β-catenin mRNA were significantly higher in older females than older males (p < 0.05). Gene expression of ActRIIB, myostatin, and TGF-β1 were higher in older adults compared to younger adults (all p < 0.05). There was, however, no difference in the total protein content of myostatin, myoD or myogenin (all p > 0.05), whereas Smad3 protein phosphorylation was 48% lower (p < 0.05) in muscle from older adults. Conclusions Increased abundance of mRNA of fibrotic markers was observed in muscle from older adults and was partly accompanied by altered abundance of pro-fibrotic ligands in a sex specific manner.

Year2017
JournalEuropean Journal of Applied Physiology
Journal citation117 (7), pp. 1463 - 1472
PublisherSpringer-Verlag
ISSN1439-6319
Digital Object Identifier (DOI)https://doi.org/10.1007/s00421-017-3639-4
Scopus EID2-s2.0-85019129561
Page range1463 - 1472
Research GroupMary MacKillop Institute for Health Research
Publisher's version
File Access Level
Controlled
Place of publicationGermany
EditorsH. Westerblad and K. R. Westerterp
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