Age and sex differences in human skeletal muscle fibrosis markers and transforming growth factor-ß signaling

Journal article


Parker, L, Caldow, Marissa K., Watts, Rani, Levinger, P., Cameron-Smith, David and Levinger, Itamar. (2017) Age and sex differences in human skeletal muscle fibrosis markers and transforming growth factor-ß signaling. European Journal of Applied Physiology. 117(7), pp. 1463 - 1472. https://doi.org/10.1007/s00421-017-3639-4
AuthorsParker, L, Caldow, Marissa K., Watts, Rani, Levinger, P., Cameron-Smith, David and Levinger, Itamar
Abstract

Purpose The aim of the study was to determine whether higher fibrosis markers in skeletal muscle of older adults are accompanied by increased expression of components of the canonical TGF-β signal transduction pathway. Methods Fourteen healthy young (21–35 years; 9 males and 5 females) and seventeen older (55–75 years; 9 males and 8 females) participants underwent vastus lateralis biopsies to determine intramuscular mRNA and protein expression of fibrogenic markers and TGF-β signaling molecules related to TGF-β1 and myostatin. Results Expression of mRNA encoding the pro-fibrotic factors; axin 2, collagen III, β-catenin and fibronectin, were all significantly higher (all p < 0.05) in the older participants (350, 170, 298, and 641%, respectively). Furthermore, axin 2 and β-catenin mRNA were significantly higher in older females than older males (p < 0.05). Gene expression of ActRIIB, myostatin, and TGF-β1 were higher in older adults compared to younger adults (all p < 0.05). There was, however, no difference in the total protein content of myostatin, myoD or myogenin (all p > 0.05), whereas Smad3 protein phosphorylation was 48% lower (p < 0.05) in muscle from older adults. Conclusions Increased abundance of mRNA of fibrotic markers was observed in muscle from older adults and was partly accompanied by altered abundance of pro-fibrotic ligands in a sex specific manner.

Year2017
JournalEuropean Journal of Applied Physiology
Journal citation117 (7), pp. 1463 - 1472
PublisherSpringer Verlag
ISSN1439-6319
Digital Object Identifier (DOI)https://doi.org/10.1007/s00421-017-3639-4
Scopus EID2-s2.0-85019129561
Page range1463 - 1472
Research GroupMary MacKillop Institute for Health Research
Place of publicationGermany
EditorsH. Westerblad and K. R. Westerterp
Permalink -

https://acuresearchbank.acu.edu.au/item/8653x/age-and-sex-differences-in-human-skeletal-muscle-fibrosis-markers-and-transforming-growth-factor-signaling

Restricted files

Publisher's version

  • 1
    total views
  • 0
    total downloads
  • 0
    views this month
  • 0
    downloads this month
These values are for the period from 19th October 2020, when this repository was created.

Export as

Related outputs

Fenugreek increases insulin-stimulated creatine content in l6C11 muscle myotubes
Tomcik, Kristyen, Smiles, William, Camera, Donny, Hugel, Helmut M., Hawley, John and Watts, Rani. (2017) Fenugreek increases insulin-stimulated creatine content in l6C11 muscle myotubes. European Journal of Nutrition. 56(3), pp. 973 - 979. https://doi.org/10.1007/s00394-015-1145-1
Effects of sugar-sweetened beverage consumption on microvascular and macrovascular function in a healthy population
Loader, Jordan, Meziat, Cindy, Watts, Rani, Lorenzen, Christian, Sigaudo-Roussel, Dominique, Stewart, Simon, Reboul, Cyril, Meyer, G. and Walther, Guillaume. (2017) Effects of sugar-sweetened beverage consumption on microvascular and macrovascular function in a healthy population. Arteriosclerosis Thrombosis and Vascular Biology. 37(6), pp. 1250 - 1260. https://doi.org/10.1161/ATVBAHA.116.308010
Myostatin inhibits proliferation and insulin-stimulated glucose uptake in mouse liver cells
Watts, Rani, Ghozlan, Mostafa, Hughey, Curtis, Johnsen, Virginia, Shearer, Jane and Hittel, Dustin. (2014) Myostatin inhibits proliferation and insulin-stimulated glucose uptake in mouse liver cells. Biochemistry and Cell Biology. 92(3), pp. 1 - 9. https://doi.org/10.1139/bcb-2014-0004
Myostatin-induced inhibition of the long noncoding RNA Malat1 is associated with decreased myogenesis
Watts, Rani, Johnsen, Virginia L., Shearer, Jane and Hittel, Dustin S.. (2013) Myostatin-induced inhibition of the long noncoding RNA Malat1 is associated with decreased myogenesis. American Journal of Physiology: Cell Physiology. 304(10), pp. C995 - C1001. https://doi.org/10.1152/ajpcell.00392.2012
Increased Smad signaling and reduced MRF expression in skeletal muscle from obese subjects
Watts, Rani, McAinch, A, Dixon, Jason, O'Brien, Paul and Cameron-Smith, David. (2013) Increased Smad signaling and reduced MRF expression in skeletal muscle from obese subjects. Obesity: a resourch journal. https://doi.org/10.1002/oby.20070
Undernutrition during suckling in rats elevates plasma adiponectin and its receptor in skeletal muscle regardless of diet composition: A protective effect?
Prior, Larissa, Velkoska, Elena, Watts, Rani, Cameron-Smith, David and Morris, Margaret J.. (2008) Undernutrition during suckling in rats elevates plasma adiponectin and its receptor in skeletal muscle regardless of diet composition: A protective effect? International Journal of Obesity. 32(10), pp. 1585 - 1594. https://doi.org/10.1038/ijo.2008.141
Brain neuropeptide Y and CCK and peripheral adipokine receptors: Temporal response in obesity induced by palatable diet
Morris, N. J., Watts, Rani, Shulkes, A. and Cameron-Smith, David. (2008) Brain neuropeptide Y and CCK and peripheral adipokine receptors: Temporal response in obesity induced by palatable diet. International Journal of Obesity. 32, pp. 249 - 258. https://doi.org/10.1038/sj.ijo.0803716