A protocol for measurement of noncoding RNA in human serum
Journal article
Taylor, Caroline J., Satoor, Sarang N., Ranjan, Amaresh K., Pereira e Cotta, Maria V. and Joglekar, Mugdha V.. (2012). A protocol for measurement of noncoding RNA in human serum. Experimental Diabetes Research. 2012, pp. 1 - 4. https://doi.org/10.1155/2012/168368
Authors | Taylor, Caroline J., Satoor, Sarang N., Ranjan, Amaresh K., Pereira e Cotta, Maria V. and Joglekar, Mugdha V. |
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Abstract | MicroRNAs (miRNAs) are small noncoding RNAs that act as regulators of gene expression by targeting mature messenger RNAs. Following the initial report of the presence of miRNAs in serum and plasma a number of studies have successfully demonstrated the use of these miRNAs as biomarkers of disease. Currently, there are many methods of isolating total RNA from liquid samples. Here, we describe a simple, cost effective method for extraction of RNA from human serum as well as subsequent real time PCR analysis of miRNA levels. |
Year | 2012 |
Journal | Experimental Diabetes Research |
Journal citation | 2012, pp. 1 - 4 |
Publisher | Hindawi Publishing Corporation |
ISSN | 1687-5214 |
Digital Object Identifier (DOI) | https://doi.org/10.1155/2012/168368 |
Scopus EID | 2-s2.0-84864928987 |
Open access | Open access |
Page range | 1 - 4 |
Research Group | Sports Performance, Recovery, Injury and New Technologies (SPRINT) Research Centre |
Publisher's version | License |
Place of publication | United States of America |
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