The muscle protein synthetic response to the combined ingestion of protein and carbohydrate is not impaired in healthy older men

Journal article


Kiskini, Alexandra, Hamer, Henrike M., Wall, Benjamin T., Groen, Bart B. L., De Lange, Anneke, Bakker, Jaap A., Senden, Joan M. G., Verdijk, Lex B. and Van Loon, Luc J.C. (2013). The muscle protein synthetic response to the combined ingestion of protein and carbohydrate is not impaired in healthy older men. Age. 35(6), pp. 2389 - 2398. https://doi.org/10.1007/s11357-013-9522-2
AuthorsKiskini, Alexandra, Hamer, Henrike M., Wall, Benjamin T., Groen, Bart B. L., De Lange, Anneke, Bakker, Jaap A., Senden, Joan M. G., Verdijk, Lex B. and Van Loon, Luc J.C
Abstract

Aging is associated with a progressive decline in skeletal muscle mass. It has been hypothesized that an attenuated muscle protein synthetic response to the main anabolic stimuli may contribute to the age-related loss of muscle tissue. The aim of the present study was to compare the muscle protein synthetic response following ingestion of a meal-like amount of dietary protein plus carbohydrate between healthy young and older men. Twelve young ( 21 ± 1 years ) and 12 older ( 75 ± 1 years ) men consumed 20 g of intrinsically l-[1-13C]phenylalanine-labeled protein with 40 g of carbohydrate. Ingestion of specifically produced intrinsically l-[1-13C]phenylalanine-labeled protein allowed us to assess the subsequent incorporation of casein-derived amino acids into muscle protein. Blood samples were collected at regular intervals, with muscle biopsies obtained prior to and 2 and 6 h after protein plus carbohydrate ingestion. The acute post-prandial rise in plasma glucose and insulin concentrations was significantly greater in the older compared with the younger males. Plasma amino acid concentrations increased rapidly following drink ingestion in both groups. However, plasma leucine concentrations were significantly lower at t = 90 min in the older when compared with the young group ( P  <  0.05 ). Muscle protein-bound l-[1-13C]phenylalanine enrichments increased to 0.0071 ± 0.0016 and 0.0072 ± 0.0013 mole percent excess ( MPE ) at 2 h and 0.0229 ± 0.0016 and 0.0213 ± 0.0024 MPE at 6 h following ingestion of the intrinsically labeled protein in the young and older males, respectively, with no differences between groups ( P  >  0.05 ). We conclude that the use of dietary protein-derived amino acids for muscle protein synthesis is not impaired in healthy older men following intake of protein plus carbohydrate.

Keywordsskeletal muscle; aging; sarcopenia; amino acids; anabolic resistance
Year2013
JournalAge
Journal citation35 (6), pp. 2389 - 2398
PublisherSpringer Netherlands
ISSN0161-9152
Digital Object Identifier (DOI)https://doi.org/10.1007/s11357-013-9522-2
Scopus EID2-s2.0-84892511955
Page range2389 - 2398
Research GroupMary MacKillop Institute for Health Research
Publisher's version
File Access Level
Controlled
Place of publicationNetherlands
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