Phosphoproteome and drug response effects mediated by the three protein phosphatase 2A inhibitor proteins CIP2A, SET, and PME-1
Journal article
Kauko, Otto, Imanishi, Susumu Y., Kulesskiy, Evgeny, Yetukuri, Laxman, Laajala, Teemu Daniel, Sharma, Mukund, Pavic, Karolina, Aakula, Anna, Rupp, Christian, Jumppanen, Mikael, Haapaniemi, Pekka, Ruan, Luyao, Yadav, Bhagwan, Suni, Veronika, Varila, Taru, Corthals, Garry L., Reimand, Jüri, Wennerberg, Krister, Aittokallio, Tero and Westermarck, Jukka. (2020). Phosphoproteome and drug response effects mediated by the three protein phosphatase 2A inhibitor proteins CIP2A, SET, and PME-1. Journal of Biological Chemistry. 295(13), pp. 4194-4211. https://doi.org/10.1074/jbc.RA119.011265
Authors | Kauko, Otto, Imanishi, Susumu Y., Kulesskiy, Evgeny, Yetukuri, Laxman, Laajala, Teemu Daniel, Sharma, Mukund, Pavic, Karolina, Aakula, Anna, Rupp, Christian, Jumppanen, Mikael, Haapaniemi, Pekka, Ruan, Luyao, Yadav, Bhagwan, Suni, Veronika, Varila, Taru, Corthals, Garry L., Reimand, Jüri, Wennerberg, Krister, Aittokallio, Tero and Westermarck, Jukka |
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Abstract | Protein phosphatase 2A (PP2A) critically regulates cell signaling and is a human tumor suppressor. PP2A complexes are modulated by proteins such as cancerous inhibitor of protein phosphatase 2A (CIP2A), protein phosphatase methylesterase 1 (PME-1), and SET nuclear proto-oncogene (SET) that often are deregulated in cancers. However, how they impact cellular phosphorylation and how redundant they are in cellular regulation is poorly understood. Here, we conducted a systematic phosphoproteomics screen for phosphotargets modulated by siRNA-mediated depletion of CIP2A, PME-1, and SET (to reactivate PP2A) or the scaffolding A-subunit of PP2A (PPP2R1A) (to inhibit PP2A) in HeLa cells. We identified PP2A-modulated targets in diverse cellular pathways, including kinase signaling, cytoskeleton, RNA splicing, DNA repair, and nuclear lamina. The results indicate nonredundancy among CIP2A, PME-1, and SET in phosphotarget regulation. Notably, PP2A inhibition or reactivation affected largely distinct phosphopeptides, introducing a concept of nonoverlapping phosphatase inhibition- and activation-responsive sites (PIRS and PARS, respectively). This phenomenon is explained by the PPP2R1A inhibition impacting primarily dephosphorylated threonines, whereas PP2A reactivation results in dephosphorylation of clustered and acidophilic sites. Using comprehensive drug-sensitivity screening in PP2A-modulated cells to evaluate the functional impact of PP2A across diverse cellular pathways targeted by these drugs, we found that consistent with global phosphoproteome effects, PP2A modulations broadly affect responses to more than 200 drugs inhibiting a broad spectrum of cancer-relevant targets. These findings advance our understanding of the phosphoproteins, pharmacological responses, and cellular processes regulated by PP2A modulation and may enable the development of combination therapies. |
Keywords | protein phosphatase 2 (PP2A); cancer; drug screening; phosphoproteomics; systems biology; AURK inhibitor; LMNA; nucleophosmin; phosphatase activation-responsive site (PARS); phosphatase inhibition-responsive site (PIRS) |
Year | 2020 |
Journal | Journal of Biological Chemistry |
Journal citation | 295 (13), pp. 4194-4211 |
Publisher | American Society for Biochemistry and Molecular Biology, Inc. |
ISSN | 0021-9258 |
Digital Object Identifier (DOI) | https://doi.org/10.1074/jbc.RA119.011265 |
Scopus EID | 2-s2.0-85082514095 |
Open access | Published as ‘gold’ (paid) open access |
Research or scholarly | Research |
Page range | 4194-4211 |
Publisher's version | License File Access Level Open |
Output status | Published |
Publication dates | |
Online | 04 Jan 2021 |
Publication process dates | |
Deposited | 04 May 2021 |
https://acuresearchbank.acu.edu.au/item/8vz56/phosphoproteome-and-drug-response-effects-mediated-by-the-three-protein-phosphatase-2a-inhibitor-proteins-cip2a-set-and-pme-1
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Publisher's version
OA_Kauko_2020_Phosphoproteome_and_drug_response_effects_mediated.pdf | |
License: CC BY 4.0 | |
File access level: Open |
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