The molecular basis of how buried human leukocyte antigen polymorphism modulates natural killer cell function
Journal article
Saunders, Philippa M., MacLachlan, Bruce J., Pymm, Phillip, Illing, Patricia T., Deng, Yuanchen, Wong, Shu Cheng, Oates, Clare V. L., Purcell, Anthony W., Rossjohn, Jamie, Vivian, Julian P. and Brooks, Andrew G.. (2020). The molecular basis of how buried human leukocyte antigen polymorphism modulates natural killer cell function. Proceedings of the National Academy of Sciences of the United States of America. 117(21), pp. 11636-11647. https://doi.org/10.1073/pnas.1920570117
Authors | Saunders, Philippa M., MacLachlan, Bruce J., Pymm, Phillip, Illing, Patricia T., Deng, Yuanchen, Wong, Shu Cheng, Oates, Clare V. L., Purcell, Anthony W., Rossjohn, Jamie, Vivian, Julian P. and Brooks, Andrew G. |
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Abstract | Micropolymorphisms within human leukocyte antigen (HLA) class I molecules can change the architecture of the peptide-binding cleft, leading to differences in peptide presentation and T cell recognition. The impact of such HLA variation on natural killer (NK) cell recognition remains unclear. Given the differential association of HLA-B*57:01 and HLA-B*57:03 with the control of HIV, recognition of these HLA-B57 allomorphs by the killer cell immunoglobulin-like receptor (KIR) 3DL1 was compared. Despite differing by only two polymorphic residues, both buried within the peptide-binding cleft, HLA-B*57:01 more potently inhibited NK cell activation. Direct-binding studies showed KIR3DL1 to preferentially recognize HLA-B*57:01, particularly when presenting peptides with positively charged position (P)Ω-2 residues. In HLA-B*57:01, charged PΩ-2 residues were oriented toward the peptide-binding cleft and away from KIR3DL1. In HLA-B*57:03, the charged PΩ-2 residues protruded out from the cleft and directly impacted KIR3DL1 engagement. Accordingly, KIR3DL1 recognition of HLA class I ligands is modulated by both the peptide sequence and conformation, as determined by the HLA polymorphic framework, providing a rationale for understanding differences in clinical associations. |
Year | 2020 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Journal citation | 117 (21), pp. 11636-11647 |
Publisher | National Academy of Sciences |
ISSN | 0027-8424 |
Digital Object Identifier (DOI) | https://doi.org/10.1073/pnas.1920570117 |
PubMed ID | 32404419 |
Scopus EID | 2-s2.0-85085489469 |
PubMed Central ID | PMC7261055 |
Page range | 11636-11647 |
Funder | National Health and Medical Research Council (NHMRC) |
Australian Research Council (ARC) | |
Publisher's version | License All rights reserved File Access Level Controlled |
Output status | Published |
Publication dates | |
Online | 13 May 2020 |
Publication process dates | |
Deposited | 28 Nov 2023 |
ARC Funded Research | This output has been funded, wholly or partially, under the Australian Research Council Act 2001 |
https://acuresearchbank.acu.edu.au/item/8zzq6/the-molecular-basis-of-how-buried-human-leukocyte-antigen-polymorphism-modulates-natural-killer-cell-function
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