Characterization of Monoclonal Antibodies to Measure Cell Surface Protein Levels of Human Interferon-Lambda Receptor 1

Journal article

de Weerd, Nicole A., Ogungbola, Olamide, Liu, Xinyun, Matthews, Antony Y., Ismail, Amina, Vivian, Julian Philip, Lim, San S., Tyrrell, D. Lorne, Putcha, Niru, Skawinski, Mike, Dickensheets, Harold, Lavoie, Thomas B., Donnelly, Raymond P., Hertzog, Paul J. and Santer, Deanna M.. (2023). Characterization of Monoclonal Antibodies to Measure Cell Surface Protein Levels of Human Interferon-Lambda Receptor 1. Journal of Interferon and Cytokine Research. 43(9), pp. 403-413. https://doi.org/10.1089/jir.2023.0040
Authorsde Weerd, Nicole A., Ogungbola, Olamide, Liu, Xinyun, Matthews, Antony Y., Ismail, Amina, Vivian, Julian Philip, Lim, San S., Tyrrell, D. Lorne, Putcha, Niru, Skawinski, Mike, Dickensheets, Harold, Lavoie, Thomas B., Donnelly, Raymond P., Hertzog, Paul J. and Santer, Deanna M.
Abstract

Type III interferons (IFN-lambdas, IFN-λs) are important antiviral cytokines that can also modulate immune responses by acting through a heterodimeric receptor composed of the specific and limited expressed IFN-λR1 chain and the ubiquitous IL-10R2 chain, which is shared with IL-10 family cytokines. Conflicting data have been reported regarding which cells express the IFN-λR1 subunit and directly respond to IFN-λs. This is, in part, owing to transcript levels of the IFN-λR1 gene, IFNLR1, not always correlating with cell surface protein levels. In this study, we tested a panel of novel monoclonal antibodies (mAbs) that specifically recognize human IFN-λR1. Initially, antigen specificity was confirmed by enzyme-linked immunosorbent assay (ELISA), from which a subset of antibodies was selected for additional flow cytometry and neutralization assays. We further characterized two antibodies based on their strong ELISA binding activity (HLR1 and HLR14) and found only HLR14 could reliably detect cell surface IFN-λR1 protein on a variety of cell lines by flow cytometry. HLR14 could also detect IFN-λR1 protein on certain primary human blood cells, including plasmacytoid dendritic cells and B cells from peripheral blood. Availability of the HLR14 mAb will enable the quantification of IFN-λR1 protein levels on cells and better characterization of the cell specificity of the IFN-λ response.

Keywordstype III interferons; flow cytometry; antiviral; IFN receptors
Year01 Jan 2023
JournalJournal of Interferon and Cytokine Research
Journal citation43 (9), pp. 403-413
PublisherMary Ann Liebert Inc
ISSN1079-9907
Digital Object Identifier (DOI)https://doi.org/10.1089/jir.2023.0040
Web address (URL)https://www.liebertpub.com/doi/10.1089/jir.2023.0040
Open accessPublished as non-open access
Research or scholarlyResearch
Page range403-413
Publisher's version
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All rights reserved
File Access Level
Controlled
Output statusPublished
Publication dates
Online15 Sep 2023
Publication process dates
Completed27 Jul 2023
Deposited12 Mar 2024
Additional information

Copyright 2023, Mary Ann Liebert, Inc., publishers.

Place of publicationUnited States
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