Engineering Cell Lines for Specific Human Leukocyte Antigen Presentation
Book chapter
Jin, Dongbin, Loh, Khai Lee, Shamekhi, Tima, Ting, Yi Tian, Lim Kam Sian, Terry C. C., Roest, James, Ooi, Joshua D., Vivian, Julian Philip and Faridi, Pouya. (2023). Engineering Cell Lines for Specific Human Leukocyte Antigen Presentation. In In Jenkins, Brendan J. (Ed.). Inflammation and Cancer : Methods and Protocols pp. 351-369 Springer - Humana Press. https://doi.org/10.1007/978-1-0716-3331-1_25
Authors | Jin, Dongbin, Loh, Khai Lee, Shamekhi, Tima, Ting, Yi Tian, Lim Kam Sian, Terry C. C., Roest, James, Ooi, Joshua D., Vivian, Julian Philip and Faridi, Pouya |
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Editors | Jenkins, Brendan J. |
Abstract | Epitope-specific immunotherapies have enabled the targeted treatment of a variety of diseases, ranging from cancer, infection, and autoimmune disorders. For CD8+ T cell-based therapies, the precise identification of immunogenic peptides presented by human leukocyte antigen (HLA) class I is essential which can be achieved by immunopeptidomics. Here, using lentivirus-mediated transduction and cell sorting approaches, we present a method to engineer a cell line that does not express its native HLA but instead expresses an HLA of interest (in this instance HLA-A*02:01). This technique can be used to elucidate the immunopeptidome of cell lines expressing different HLAs. |
Keywords | Cancer; Cell lines Gene editing HLA Immunopeptidomics Lentivirus Transduction Immunotherapy Neoantigens; Gene editing; HLA; Immunopeptidomics; Lentivirus; Transduction; Immunotherapy; Neoantigens |
Page range | 351-369 |
Year | 01 Jan 2023 |
Book title | Inflammation and Cancer : Methods and Protocols |
Publisher | Springer - Humana Press |
Place of publication | United States |
Edition | 2 |
Series | Methods in Molecular Biology (MIMB,volume 2691) |
ISBN | 978-1-0716-3330-4 |
ISSN | 1064-3745 |
Digital Object Identifier (DOI) | https://doi.org/10.1007/978-1-0716-3331-1_25 |
Web address (URL) | https://link.springer.com/protocol/10.1007/978-1-0716-3331-1_25 |
Open access | Published as non-open access |
Research or scholarly | Research |
Publisher's version | License All rights reserved File Access Level Controlled |
Output status | Published |
Publication dates | |
24 Jun 2023 | |
Publication process dates | |
Accepted | Jun 2023 |
Deposited | 10 Apr 2024 |
Additional information | © 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. |
https://acuresearchbank.acu.edu.au/item/904xz/engineering-cell-lines-for-specific-human-leukocyte-antigen-presentation
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