Engineering Cell Lines for Specific Human Leukocyte Antigen Presentation

Book chapter

Jin, Dongbin, Loh, Khai Lee, Shamekhi, Tima, Ting, Yi Tian, Lim Kam Sian, Terry C. C., Roest, James, Ooi, Joshua D., Vivian, Julian Philip and Faridi, Pouya. (2023). Engineering Cell Lines for Specific Human Leukocyte Antigen Presentation. In In Jenkins, Brendan J. (Ed.). Inflammation and Cancer : Methods and Protocols pp. 351-369 Springer - Humana Press. https://doi.org/10.1007/978-1-0716-3331-1_25
AuthorsJin, Dongbin, Loh, Khai Lee, Shamekhi, Tima, Ting, Yi Tian, Lim Kam Sian, Terry C. C., Roest, James, Ooi, Joshua D., Vivian, Julian Philip and Faridi, Pouya
EditorsJenkins, Brendan J.
Abstract

Epitope-specific immunotherapies have enabled the targeted treatment of a variety of diseases, ranging from cancer, infection, and autoimmune disorders. For CD8+ T cell-based therapies, the precise identification of immunogenic peptides presented by human leukocyte antigen (HLA) class I is essential which can be achieved by immunopeptidomics. Here, using lentivirus-mediated transduction and cell sorting approaches, we present a method to engineer a cell line that does not express its native HLA but instead expresses an HLA of interest (in this instance HLA-A*02:01). This technique can be used to elucidate the immunopeptidome of cell lines expressing different HLAs.

KeywordsCancer; Cell lines Gene editing HLA Immunopeptidomics Lentivirus Transduction Immunotherapy Neoantigens; Gene editing; HLA; Immunopeptidomics; Lentivirus; Transduction; Immunotherapy; Neoantigens
Page range351-369
Year01 Jan 2023
Book titleInflammation and Cancer : Methods and Protocols
PublisherSpringer - Humana Press
Place of publicationUnited States
Edition2
SeriesMethods in Molecular Biology (MIMB,volume 2691)
ISBN978-1-0716-3330-4
ISSN1064-3745
Digital Object Identifier (DOI)https://doi.org/10.1007/978-1-0716-3331-1_25
Web address (URL)https://link.springer.com/protocol/10.1007/978-1-0716-3331-1_25
Open accessPublished as non-open access
Research or scholarlyResearch
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Controlled
Output statusPublished
Publication dates
Print24 Jun 2023
Publication process dates
AcceptedJun 2023
Deposited10 Apr 2024
Additional information

© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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