MRT letter: Micro-to nanoscale sample collection for high throughput microscopy
Journal article
Cheong, Brandon Huey-Ping, Liew, Oi Wah and Ng, Tuck Wah. (2013). MRT letter: Micro-to nanoscale sample collection for high throughput microscopy. Microscopy Research and Technique. 76(8), pp. 767 - 773. https://doi.org/10.1002/jemt.22238
Authors | Cheong, Brandon Huey-Ping, Liew, Oi Wah and Ng, Tuck Wah |
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Abstract | In high throughput microscopy, it is often assumed that the objects under investigation are fixed spatially. In addition, it is also presumed that the objects are sufficiently populated, otherwise there will be need to search through vast tracks of field of views before any recording can be done. The ability to collect objects at one location in the hydrated state is thus desirable and this is a challenge when the density of target objects in a sample is very low. In this work, we report that the generation of a squeezing flow from a circular coverslip compressing on suspensions is able to collect particulate (microbeads, fluorescent nanobeads and live algal cells) and non‐particulate (EGFP) objects at the rim region of the coverslip. With a coverslip of 13 mm diameter, volumes between 2 µL and 4 µL were found to completely fill the coverslip without breaching the rims. Sample compression speeds between 100 µm/s and 1000 µm/s did not have any effect on object collection outcomes. In effect, the simple placement of coverslips on top the drop of sample by hand without a motorized translator was found to produce similar collection outcomes. Quantitative measurements confirmed that all the objects investigated were displaced and relocated at the rim regions to a very high degree. Microsc. Res. Tech. 76:767–773, 2013. © 2013 Wiley Periodicals, Inc. |
Keywords | high throughput microscopy; object collection; coverslips; squeeze flow |
Year | 2013 |
Journal | Microscopy Research and Technique |
Journal citation | 76 (8), pp. 767 - 773 |
Publisher | John Wiley & Sons |
ISSN | 1059-910X |
Digital Object Identifier (DOI) | https://doi.org/10.1002/jemt.22238 |
Scopus EID | 2-s2.0-84880704077 |
Page range | 767 - 773 |
Research Group | School of Behavioural and Health Sciences |
Publisher's version | File Access Level Controlled |
Place of publication | United States of America |
https://acuresearchbank.acu.edu.au/item/86504/mrt-letter-micro-to-nanoscale-sample-collection-for-high-throughput-microscopy
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