A colorimetric-based accurate method for the determination of enterovirus 71 titer
Journal article
Pourianfar, Hamid Reza, Javadi, Arman and Grollo, Lara. (2012). A colorimetric-based accurate method for the determination of enterovirus 71 titer. Indian Journal of Virology. 23(3), pp. 303 - 310. https://doi.org/10.1007/s13337-012-0105-0
Authors | Pourianfar, Hamid Reza, Javadi, Arman and Grollo, Lara |
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Abstract | The 50 % tissue culture infectious dose (TCID50) is still one of the most commonly used techniques for estimating virus titers. However, the traditional TCID50 assay is time consuming, susceptible to subjective errors and generates only quantal data. Here, we describe a colorimetric-based approach for the titration of Enterovirus 71 (EV71) using a modified method for making virus dilutions. In summary, the titration of EV71 using MTT or MTS staining with a modified virus dilution method decreased the time of the assay and eliminated the subjectivity of observational results, improving accuracy, reproducibility and reliability of virus titration, in comparison with the conventional TCID50 approach (p < 0.01). In addition, the results provided evidence that there was better correlation between a plaquing assay and our approach when compared to the traditional TCID50 approach. This increased accuracy also improved the ability to predict the number of virus plaque forming units present in a solution. These improvements could be of use for any virological experimentation, where a quick accurate titration of a virus capable of causing cell destruction is required or a sensible estimation of the number of viral plaques based on TCID50 of a virus is desired. |
Keywords | enterovirus 71; MTS; MTT; TCID50; virus titration |
Year | 2012 |
Journal | Indian Journal of Virology |
Journal citation | 23 (3), pp. 303 - 310 |
ISSN | 0970-2822 |
Digital Object Identifier (DOI) | https://doi.org/10.1007/s13337-012-0105-0 |
Scopus EID | 2-s2.0-84879550666 |
Page range | 303 - 310 |
Research Group | School of Behavioural and Health Sciences |
Publisher's version | File Access Level Controlled |
https://acuresearchbank.acu.edu.au/item/8q0q9/a-colorimetric-based-accurate-method-for-the-determination-of-enterovirus-71-titer
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