MitoQ supplementation augments acute exercise-induced increases in muscle PGC1α mRNA and improves training-induced increases in peak power independent of mitochondrial content and function in untrained middle-aged men

Journal article


Broome, S. C., Pham, T., Braakhuis, A. J., Narang, R., Wang, H. W., Hickey, A. J. R., Mitchell, C. J. and Merry, T. L.. (2022). MitoQ supplementation augments acute exercise-induced increases in muscle PGC1α mRNA and improves training-induced increases in peak power independent of mitochondrial content and function in untrained middle-aged men. Redox Biology. 53, p. Article 102341. https://doi.org/10.1016/j.redox.2022.102341
AuthorsBroome, S. C., Pham, T., Braakhuis, A. J., Narang, R., Wang, H. W., Hickey, A. J. R., Mitchell, C. J. and Merry, T. L.
Abstract

The role of mitochondrial ROS in signalling muscle adaptations to exercise training has not been explored in detail. We investigated the effect of supplementation with the mitochondria-targeted antioxidant MitoQ on a) the skeletal muscle mitochondrial and antioxidant gene transcriptional response to acute high-intensity exercise and b) skeletal muscle mitochondrial content and function following exercise training. In a randomised, double-blind, placebo-controlled, parallel design study, 23 untrained men (age: 44 ± 7 years, VO2peak: 39.6 ± 7.9 ml/kg/min) were randomised to receive either MitoQ (20 mg/d) or a placebo for 10 days before completing a bout of high-intensity interval exercise (cycle ergometer, 10 × 60 s at VO2peak workload with 75 s rest). Blood samples and vastus lateralis muscle biopsies were collected before exercise and immediately and 3 h after exercise. Participants then completed high-intensity interval training (HIIT; 3 sessions per week for 3 weeks) and another blood sample and muscle biopsy were collected. There was no effect of acute exercise or MitoQ on systemic (plasma protein carbonyls and reduced glutathione) or skeletal muscle (mtDNA damage and 4-HNE) oxidative stress biomarkers. Acute exercise-induced increases in skeletal muscle peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1-α) mRNA expression were augmented in the MitoQ group. Despite this, training-induced increases in skeletal muscle mitochondrial content were similar between groups. HIIT-induced increases in VO2peak and 20 km time trial performance were also similar between groups while training-induced increases in peak power achieved during the VO2peak test were augmented in the MitoQ group. These data suggest that training-induced increases in peak power are enhanced following MitoQ supplementation, which may be related to the augmentation of skeletal muscle PGC1α expression following acute exercise. However, these effects do not appear to be related to an effect of MitoQ supplementation on exercise-induced oxidative stress or training-induced mitochondrial biogenesis in skeletal muscle.

Keywordsmitochondria; antioxidant; exercise; ROS; adaptation; performance
Year2022
JournalRedox Biology
Journal citation53, p. Article 102341
PublisherElsevier B.V.
ISSN2213-2317
Digital Object Identifier (DOI)https://doi.org/10.1016/j.redox.2022.102341
PubMed ID35623315
Scopus EID2-s2.0-85131077547
PubMed Central IDPMC9142706
Open accessPublished as ‘gold’ (paid) open access
Page range1-11
FunderCallaghan Innovation
MitoQ
Publisher's version
License
File Access Level
Open
Output statusPublished
Publication dates
Online20 May 2022
Publication process dates
Accepted14 May 2022
Deposited17 Jan 2023
Permalink -

https://acuresearchbank.acu.edu.au/item/8y9q0/mitoq-supplementation-augments-acute-exercise-induced-increases-in-muscle-pgc1-mrna-and-improves-training-induced-increases-in-peak-power-independent-of-mitochondrial-content-and-function-in

Download files


Publisher's version
  • 20
    total views
  • 7
    total downloads
  • 0
    views this month
  • 0
    downloads this month
These values are for the period from 19th October 2020, when this repository was created.

Export as

Related outputs

A role for β-catenin in diet-induced skeletal muscle insulin resistance
Mason, Stewart W. C., Dissanayake, Waruni C., Broome, Sophie C., Hedges, Christopher P., Peters, Wouter M., Gram, Martin, Rowlands, David S., Shepherd, Peter R. and Merry, Troy L.. (2023). A role for β-catenin in diet-induced skeletal muscle insulin resistance. Physiological Reports. 11(4), p. Article e15536. https://doi.org/10.14814/phy2.15536
Mitochondria-targeted antioxidant supplementation does not affect muscle soreness or recovery of maximal voluntary isometric contraction force following muscle-damaging exercise in untrained men : A randomized clinical trial
Broome, S. C., Atiola, R. D., Braakhuis, A. J., Mitchell, C. J. and Merry, T. L.. (2022). Mitochondria-targeted antioxidant supplementation does not affect muscle soreness or recovery of maximal voluntary isometric contraction force following muscle-damaging exercise in untrained men : A randomized clinical trial. Applied Physiology, Nutrition and Metabolism. 47(7), pp. 762-774. https://doi.org/10.1139/apnm-2021-0767
Skeletal muscle NOX4 is required for adaptive responses that prevent insulin resistance
Xirouchaki, Chrysovalantou E., Jia, Yaoyao, McGrath, Meagan J., Greatorex, Spencer, Tran, Melanie, Merry, Troy L., Hong, Dawn, Eramo, Matthew J., Broome, Sophie C., Woodhead, Jonathan S. T., D'souza, Randall F., Gallagher, Jenny, Salimova, Ekaterina, Huang, Cheng, Schittenhelm, Ralf B., Sadoshima, Junichi, Watt, Matthew J., Mitchell, Christina A. and Tiganis, Tony. (2021). Skeletal muscle NOX4 is required for adaptive responses that prevent insulin resistance. Science Advances. 7(51), p. Article eabl4988. https://doi.org/10.1126/sciadv.abl4988
β-Catenin is required for optimal exercise- andcontraction-stimulated skeletal muscle glucose uptake
Masson, Stewart W. C., Woodhead, Jonathan S. T., D'Souza, Randall F., Broome, Sophie C., MacRae, Caitlin, Cho, Hyun C., Atiola, Robert D., Futi, Tumani, Dent, Jessica R., Shepherd, Peter R. and Merry, Troy L.. (2021). β-Catenin is required for optimal exercise- andcontraction-stimulated skeletal muscle glucose uptake. Journal of Physiology. 599(16), pp. 3897-3912. https://doi.org/10.1113/JP281352
Pre-exercise carbohydrate or protein ingestion influences substrate oxidation but not performance or hunger compared with cycling in the fasted state
Rothschild, Jeffrey A., Kilding, Andrew E., Broome, Sophie C., Stewart, Tom, Cronin, John B. and Plews, Daniel J.. (2021). Pre-exercise carbohydrate or protein ingestion influences substrate oxidation but not performance or hunger compared with cycling in the fasted state. Nutrients. 13(4), p. Article 1291. https://doi.org/10.3390/nu13041291
Mitochondria-targeted antioxidantsupplementation improves 8 km time trialperformance in middle-aged trained male cyclists
Broome, S. C., Braakhuis, A. J., Mitchell, C. J. and Merry, T. L.. (2021). Mitochondria-targeted antioxidantsupplementation improves 8 km time trialperformance in middle-aged trained male cyclists. Journal of the International Society of Sports Nutrition. 18(1), p. Article 58. https://doi.org/10.1186/s12970-021-00454-0
MitoQ and CoQ10 supplementation mildly suppresses skeletal muscle mitochondrial hydrogen peroxide levels without impacting mitochondrial function in middle‑aged men
Pham, Toan, MacRae, Caitlin L., Broome, Sophie C., D'Souza, Randall F., Narang, Ravi, Wang, Hsiang W., Mori, Trevor A., Hickey, Anthony J. R., Mitchell, Cameron J. and Merry, Troy L.. (2020). MitoQ and CoQ10 supplementation mildly suppresses skeletal muscle mitochondrial hydrogen peroxide levels without impacting mitochondrial function in middle‑aged men. European Journal of Applied Physiology. 120, pp. 1657-1669. https://doi.org/10.1007/s00421-020-04396-4
Mitochondria-targeted antioxidants and skeletal muscle function
Broome, Sophie, Woodhead, Jonathan and Merry, Troy. (2018). Mitochondria-targeted antioxidants and skeletal muscle function. Antioxidants. 7(8), p. Article 107. https://doi.org/10.3390/antiox7080107