MitoQ supplementation augments acute exercise-induced increases in muscle PGC1α mRNA and improves training-induced increases in peak power independent of mitochondrial content and function in untrained middle-aged men

Journal article


Broome, S. C., Pham, T., Braakhuis, A. J., Narang, R., Wang, H. W., Hickey, A. J. R., Mitchell, C. J. and Merry, T. L.. (2022). MitoQ supplementation augments acute exercise-induced increases in muscle PGC1α mRNA and improves training-induced increases in peak power independent of mitochondrial content and function in untrained middle-aged men. Redox Biology. 53, p. Article 102341. https://doi.org/10.1016/j.redox.2022.102341
AuthorsBroome, S. C., Pham, T., Braakhuis, A. J., Narang, R., Wang, H. W., Hickey, A. J. R., Mitchell, C. J. and Merry, T. L.
Abstract

The role of mitochondrial ROS in signalling muscle adaptations to exercise training has not been explored in detail. We investigated the effect of supplementation with the mitochondria-targeted antioxidant MitoQ on a) the skeletal muscle mitochondrial and antioxidant gene transcriptional response to acute high-intensity exercise and b) skeletal muscle mitochondrial content and function following exercise training. In a randomised, double-blind, placebo-controlled, parallel design study, 23 untrained men (age: 44 ± 7 years, VO2peak: 39.6 ± 7.9 ml/kg/min) were randomised to receive either MitoQ (20 mg/d) or a placebo for 10 days before completing a bout of high-intensity interval exercise (cycle ergometer, 10 × 60 s at VO2peak workload with 75 s rest). Blood samples and vastus lateralis muscle biopsies were collected before exercise and immediately and 3 h after exercise. Participants then completed high-intensity interval training (HIIT; 3 sessions per week for 3 weeks) and another blood sample and muscle biopsy were collected. There was no effect of acute exercise or MitoQ on systemic (plasma protein carbonyls and reduced glutathione) or skeletal muscle (mtDNA damage and 4-HNE) oxidative stress biomarkers. Acute exercise-induced increases in skeletal muscle peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1-α) mRNA expression were augmented in the MitoQ group. Despite this, training-induced increases in skeletal muscle mitochondrial content were similar between groups. HIIT-induced increases in VO2peak and 20 km time trial performance were also similar between groups while training-induced increases in peak power achieved during the VO2peak test were augmented in the MitoQ group. These data suggest that training-induced increases in peak power are enhanced following MitoQ supplementation, which may be related to the augmentation of skeletal muscle PGC1α expression following acute exercise. However, these effects do not appear to be related to an effect of MitoQ supplementation on exercise-induced oxidative stress or training-induced mitochondrial biogenesis in skeletal muscle.

Keywordsmitochondria; antioxidant; exercise; ROS; adaptation; performance
Year2022
JournalRedox Biology
Journal citation53, p. Article 102341
PublisherElsevier B.V.
ISSN2213-2317
Digital Object Identifier (DOI)https://doi.org/10.1016/j.redox.2022.102341
PubMed ID35623315
Scopus EID2-s2.0-85131077547
PubMed Central IDPMC9142706
Open accessPublished as ‘gold’ (paid) open access
Page range1-11
FunderCallaghan Innovation
MitoQ
Publisher's version
License
File Access Level
Open
Output statusPublished
Publication dates
Online20 May 2022
Publication process dates
Accepted14 May 2022
Deposited17 Jan 2023
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