Dehydroepiandrosterone Supplementation Results in Varying Tissue-specific Levels of Dihydrotestosterone in Male Mice
Journal article
Collden, Hannah, Nilsson, Maria, Norlen, Anna-Karin, Landin, Andreas, Windahl, Sara, Wu, Jianyao, Horkeby, Karin, Lagerquist, Marie, Ryberg, Henrik, Poutanen, Matti, Vandenput, Liesbeth and Ohlsson, Claes. (2022). Dehydroepiandrosterone Supplementation Results in Varying Tissue-specific Levels of Dihydrotestosterone in Male Mice. Endocrinology. 163(12), pp. 1-7. https://doi.org/10.1210/endocr/bqac163
Authors | Collden, Hannah, Nilsson, Maria, Norlen, Anna-Karin, Landin, Andreas, Windahl, Sara, Wu, Jianyao, Horkeby, Karin, Lagerquist, Marie, Ryberg, Henrik, Poutanen, Matti, Vandenput, Liesbeth and Ohlsson, Claes |
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Abstract | Dehydroepiandrosterone (DHEA), an adrenal androgen precursor, can be metabolized in target tissues into active sex steroids. It has been proposed that DHEA supplementation might result in restoration of physiological local sex steroid levels, but knowledge on the effect of DHEA treatment on local sex steroid levels in multiple tissues is lacking. To determine the effects of DHEA on tissue-specific levels of sex steroids, we treated orchiectomized (ORX) male mice with DHEA for 3 weeks and compared them with vehicle-treated ORX mice and gonadal intact mice. Intra-tissue levels of sex steroids were analyzed in reproductive organs (seminal vesicles, prostate, m. levator ani), major body compartments (white adipose tissue, skeletal muscle, and brain), adrenals, liver, and serum using a sensitive and validated gas chromatography-mass spectrometry method. DHEA treatment restored levels of both testosterone (T) and dihydrotestosterone (DHT) to approximately physiological levels in male reproductive organs. In contrast, this treatment did not increase DHT levels in skeletal muscle or brain. In the liver, DHEA treatment substantially increased levels of T (at least 4-fold) and DHT (+536%, P < 0.01) compared with vehicle-treated ORX mice. In conclusion, we provide a comprehensive map of the effect of DHEA treatment on intra-tissue sex steroid levels in ORX mice with a restoration of physiological levels of androgens in male reproductive organs while DHT levels were not restored in the skeletal muscle or brain. This, and the unexpected supraphysiological androgen levels in the liver, may be a cause for concern considering the uncontrolled use of DHEA. |
Keywords | androgens; dehydroepiandrosterone; dihydrotestosterone; intracrinology; mice; reproductive organs |
Year | 01 Jan 2022 |
Journal | Endocrinology |
Journal citation | 163 (12), pp. 1-7 |
Publisher | Oxford University Press |
ISSN | 0013-7227 |
Digital Object Identifier (DOI) | https://doi.org/10.1210/endocr/bqac163 |
PubMed ID | 36201601 |
Scopus EID | 2-s2.0-8514434961 |
PubMed Central ID | PMC9588255 |
Web address (URL) | https://academic.oup.com/endo/article/163/12/bqac163/6750032 |
Open access | Published as green open access |
Research or scholarly | Research |
Page range | 1-7 |
Publisher's version | License File Access Level Open |
Output status | Published |
Publication dates | |
23 Oct 2022 | |
Publication process dates | |
Accepted | 30 Sep 2022 |
Deposited | 17 Jan 2023 |
Additional information | © The Author(s) 2022. Published by Oxford University Press on behalf of the Endocrine Society. |
Place of publication | United States |
https://acuresearchbank.acu.edu.au/item/8y943/dehydroepiandrosterone-supplementation-results-in-varying-tissue-specific-levels-of-dihydrotestosterone-in-male-mice
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Publisher's version
OA_Colld_2022_Dehydroepiandrosterone_supplementation_results_in_varying_tissue.pdf | |
License: CC BY-NC-ND 4.0 | |
File access level: Open |
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