Synergy and oxygen adaptation for development of next-generation probiotics
Journal article
Khan, Muhammad Tanweer, Dwibedi, Chinmay, Sundh, Daniel, Pradhan, Meenakshi, Kraft, Jamie D., Caesar, Robert, Tremaroli, Valentina, Lorentzon, Mattias and Bäckhed, Fredrik. (2023). Synergy and oxygen adaptation for development of next-generation probiotics. Nature. 620, pp. 381-385. https://doi.org/10.1038/s41586-023-06378-w
Authors | Khan, Muhammad Tanweer, Dwibedi, Chinmay, Sundh, Daniel, Pradhan, Meenakshi, Kraft, Jamie D., Caesar, Robert, Tremaroli, Valentina, Lorentzon, Mattias and Bäckhed, Fredrik |
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Abstract | The human gut microbiota has gained interest as an environmental factor that may contribute to health or disease1. The development of next-generation probiotics is a promising strategy to modulate the gut microbiota and improve human health; however, several key candidate next-generation probiotics are strictly anaerobic2 and may require synergy with other bacteria for optimal growth. Faecalibacterium prausnitzii is a highly prevalent and abundant human gut bacterium associated with human health, but it has not yet been developed into probiotic formulations2. Here we describe the co-isolation of F. prausnitzii and Desulfovibrio piger, a sulfate-reducing bacterium, and their cross-feeding for growth and butyrate production. To produce a next-generation probiotic formulation, we adapted F. prausnitzii to tolerate oxygen exposure, and, in proof-of-concept studies, we demonstrate that the symbiotic product is tolerated by mice and humans (ClinicalTrials.gov identifier: NCT03728868) and is detected in the human gut in a subset of study participants. Our study describes a technology for the production of next-generation probiotics based on the adaptation of strictly anaerobic bacteria to tolerate oxygen exposures without a reduction in potential beneficial properties. Our technology may be used for the development of other strictly anaerobic strains as next-generation probiotics. |
Year | 2023 |
Journal | Nature |
Journal citation | 620, pp. 381-385 |
Publisher | Nature Publishing Group |
ISSN | 0028-0836 |
Digital Object Identifier (DOI) | https://doi.org/10.1038/s41586-023-06378-w |
PubMed ID | 37532933 |
Scopus EID | 2-s2.0-85166553228 |
PubMed Central ID | PMC10412450 |
Open access | Published as ‘gold’ (paid) open access |
Page range | 381-385 |
Publisher's version | License File Access Level Open |
Output status | Published |
Publication dates | |
Online | 02 Aug 2023 |
Publication process dates | |
Accepted | 27 Jun 2023 |
Deposited | 02 Apr 2025 |
Additional information | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
https://acuresearchbank.acu.edu.au/item/918q8/synergy-and-oxygen-adaptation-for-development-of-next-generation-probiotics
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OA_Khan_2023_Synergy_and_oxygen_adaptation_for_development.pdf | |
License: CC BY 4.0 | |
File access level: Open |
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