Effect of exercise on the skeletal muscle proteome in patients with type 2 diabetes

Journal article


Hussey, Sophie E., Sharoff, Carrie G., Garnham, Andrew, Yi, Zhengping, Bowen, Benjamin P., Mandarino, Lawrence J. and Hargreaves, Mark 2013. Effect of exercise on the skeletal muscle proteome in patients with type 2 diabetes. Medicine and Science in Sports and Exercise. 45 (6), pp. 1069 - 1076. https://doi.org/10.1249/MSS.0b013e3182814917
AuthorsHussey, Sophie E., Sharoff, Carrie G., Garnham, Andrew, Yi, Zhengping, Bowen, Benjamin P., Mandarino, Lawrence J. and Hargreaves, Mark
Abstract

HUSSEY, S. E., C. G. SHAROFF, A. GARNHAM, Z. YI , B. P. BOWEN, L. J. MANDARINO, and M. HARGREAVES. Effect of Exercise on the Skeletal Muscle Proteome in Patients with Type 2 Diabetes. Med. Sci. Sports Exerc., Vol. 45, No. 6, pp. 1069–1076, 2013. Purpose: Exercise training alters protein abundance in the muscle of healthy individuals, but the effect of exercise on these proteins in patients with type 2 diabetes (T2D) is unknown. The aim of this study was to determine how exercise training alters the skeletal muscle proteome in patients with T2D. Methods: Biopsies of the vastus lateralis were obtained before and after 4 wk of exercise training in six patients with T2D (54 T 4 yr; body mass index (BMI), 29 T 2) and six age- and BMI-matched control subjects (48 T 2; BMI, 28 T 3) studied at the baseline. The proteins were identified and quantified using normalized spectral abundance factors by multidimensional high-resolution mass spectrometry. Results: Of the 1329 proteins assigned at the baseline, 438 were present in at least half of all the muscle samples; of these, 15 proteins differed significantly between the patients with T2D and control subjects (P G 0.05). In the diabetic patients, the exercise training altered the abundance of 17 proteins (P G 0.05). Key training adaptations included an increase in proteins of the malate–aspartate shuttle and citric acid cycle, reduced the abundance of glycolytic proteins, and altered the abundance of cytoskeleton proteins. Conclusion: The data from this study support the ability of exercise training to alter the abundance of proteins that regulate metabolism and cytoskeletal structure in patients with T2D. These findings open new avenues for future research. Key Words: PROTEOME, EXERCISE, SKELETAL MUSCLE, INSULIN RESISTANCE

KeywordsProteome; Exercise; Skeletal Muscle; Insulin Resistance
Year2013
JournalMedicine and Science in Sports and Exercise
Journal citation45 (6), pp. 1069 - 1076
PublisherLippincott Williams & Wilkins Ltd.
ISSN0195-9131
Digital Object Identifier (DOI)https://doi.org/10.1249/MSS.0b013e3182814917
Scopus EID2-s2.0-84878918595
Page range1069 - 1076
Place of publicationUnited States
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