Effect of inorganic nitrate on exercise capacity, mitochondria respiration, and vascular function in heart failure with reduced ejection fraction
Journal article
Woessner, Mary N., Neil, Christopher, Saner, Nicholas J., Goodman, Craig A., McIlvenna, Luke C., Ortiz de Zevallos, Joaquin, Garnham, Andrew, Levinger, Itamar and Allen, Jason D.. (2020). Effect of inorganic nitrate on exercise capacity, mitochondria respiration, and vascular function in heart failure with reduced ejection fraction. Journal of Applied Physiology. 128(5), pp. 1355-1364. https://doi.org/10.1152/japplphysiol.00850.2019
Authors | Woessner, Mary N., Neil, Christopher, Saner, Nicholas J., Goodman, Craig A., McIlvenna, Luke C., Ortiz de Zevallos, Joaquin, Garnham, Andrew, Levinger, Itamar and Allen, Jason D. |
---|---|
Abstract | Chronic underperfusion of the skeletal muscle tissues is a contributor to a decrease in exercise capacity in patients with heart failure with reduced ejection fraction (HFrEF). This underperfusion is due, at least in part, to impaired nitric oxide (NO) bioavailability. Oral inorganic nitrate supplementation increases NO bioavailability and may be used to improve exercise capacity, vascular function, and mitochondrial respiration. Sixteen patients with HFrEF (fifteen men, 63 ± 4 yr, body mass index: 31.8 ± 2.1 kg/m2) participated in a randomized, double-blind, crossover design study. Following consumption of either nitrate-rich beetroot juice (16 mmol nitrate/day) or a nitrate-depleted placebo for 5 days, participants completed separate visits for assessment of exercise capacity, endothelial function, and muscle mitochondrial respiration. Participants then had a 2-wk washout before completion of the same protocol with the other intervention. Statistical significance was set a priori at P < 0.05, and between-treatment differences were analyzed via paired t test analysis. Following nitrate supplementation, both plasma nitrate and nitrite increased (933%, P < 0.001 and 94%, P < 0.05, respectively). No differences were observed for peak oxygen consumption (nitrate: 18.5 ± 1.4 mL·kg−1·min−1, placebo: 19.3 ± 1.4 mL·kg−1·min−1; P = 0.13) or time to exhaustion (nitrate: 1,165 ± 92 s, placebo: 1,207 ± 96 s; P = 0.16) following supplementation. There were no differences between interventions for measures of vascular function, mitochondrial respiratory function, or protein expression (all P > 0.05). Inorganic nitrate supplementation did not improve exercise capacity and skeletal muscle mitochondrial respiratory function in HFrEF. Future studies should explore alternative interventions to improve peripheral muscle tissue function in HFrEF. |
Keywords | beetroot juice; exercise capacity; nitrate-nitrite-NO pathway; nitric oxide |
Year | 2020 |
Journal | Journal of Applied Physiology |
Journal citation | 128 (5), pp. 1355-1364 |
Publisher | American Physiological Society |
ISSN | 8750-7587 |
Digital Object Identifier (DOI) | https://doi.org/10.1152/japplphysiol.00850.2019 |
Scopus EID | 2-s2.0-85084380068 |
Research or scholarly | Research |
Page range | 1355-1364 |
Publisher's version | License All rights reserved File Access Level Controlled |
Output status | Published |
Publication dates | |
01 May 2020 | |
Online | 07 May 2020 |
Publication process dates | |
Accepted | 12 Mar 2020 |
Deposited | 14 Apr 2021 |
https://acuresearchbank.acu.edu.au/item/8vwz6/effect-of-inorganic-nitrate-on-exercise-capacity-mitochondria-respiration-and-vascular-function-in-heart-failure-with-reduced-ejection-fraction
Restricted files
Publisher's version
106
total views0
total downloads0
views this month0
downloads this month