Acute low-intensity cycling with blood-flow restriction has no effect on metabolic signaling in human skeletal muscle compared to traditional exercise
Journal article
Smiles, William, Conceição, Miguel S., Telles, Guilherme D., Chacon-Mikahil, Mara P.T., Cavaglieri, Cláudia R., Vechin, Felipe C., Libardi, Cleiton A., Hawley, John and Camera, Donny. (2017) Acute low-intensity cycling with blood-flow restriction has no effect on metabolic signaling in human skeletal muscle compared to traditional exercise. European Journal of Applied Physiology. 117(2), pp. 345 - 358. https://doi.org/10.1007/s00421-016-3530-8
Authors | Smiles, William, Conceição, Miguel S., Telles, Guilherme D., Chacon-Mikahil, Mara P.T., Cavaglieri, Cláudia R., Vechin, Felipe C., Libardi, Cleiton A., Hawley, John and Camera, Donny |
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Abstract | Purpose. Autophagy is an intracellular degradative system sensitive to hypoxia and exercise-induced perturbations to cellular bioenergetics. We determined the effects of low-intensity endurance-based exercise performed with blood-flow restriction (BFR) on cell signaling adaptive responses regulating autophagy and substrate metabolism in human skeletal muscle. Methods In a randomized cross-over design, nine young, healthy but physically inactive males completed three experimental trials separated by 1 week of recovery con-sisting of either a resistance exercise bout (REX: 4 × 10 leg press repetitions, 70% 1-RM), endurance exercise (END: 30 min cycling, 70% VO2peak), or low-intensity cycling with BFR (15 min, 40% VO2peak). A resting muscle biopsy was obtained from the vastus lateralis 2 weeks prior to the first exercise trial and 3 h after each exercise bout. Results END increased ULK1Ser757 phosphorylation above rest and BFR (~37 to 51%, P < 0.05). Following REX, there were significant elevations compared to rest (~348%) and BFR (~973%) for p38γ MAPKThr180/Tyr182 phosphorylation (P < 0.05). Parkin content was lower fol-lowing BFR cycling compared to REX (~20%, P < 0.05). There were no exercise-induced changes in select markers of autophagy following BFR. Genes implicated in sub-strate metabolism (HK2 and PDK4) were increased above rest (~143 to 338%) and BFR cycling (~212 to 517%) with END (P < 0.001). Conclusion A single bout of low-intensity cycling with BFR is insufficient to induce intracellular “stress” responses (e.g., high rates of substrate turnover and local hypoxia) necessary to activate skeletal muscle autophagy signaling. |
Keywords | Blood-flow restriction; exercise; autophagy; mitophagy |
Year | 2017 |
Journal | European Journal of Applied Physiology |
Journal citation | 117 (2), pp. 345 - 358 |
Publisher | Springer |
ISSN | 1439-6319 |
Digital Object Identifier (DOI) | https://doi.org/10.1007/s00421-016-3530-8 |
Scopus EID | 2-s2.0-85010791363 |
Page range | 345 - 358 |
Research Group | Mary MacKillop Institute for Health Research |
Publisher's version | File Access Level Controlled |
Place of publication | Germany |
Editors | H. Westerblad and K. R. Westerterp |
https://acuresearchbank.acu.edu.au/item/8q3q6/acute-low-intensity-cycling-with-blood-flow-restriction-has-no-effect-on-metabolic-signaling-in-human-skeletal-muscle-compared-to-traditional-exercise
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