Resistance exercise augments postprandial overnight muscle protein synthesis rates
Trommelen, Jorn, Holwerda, Andrew M., Kouw, Imre W. K., Langer, Henning, Halson, Shona L., Verdijk, Lex B. and van Loon, Luc J. C.. (2016) Resistance exercise augments postprandial overnight muscle protein synthesis rates. Medicine and Science in Sports and Exercise. 48(12), pp. 2517-2525. https://doi.org/10.1249/MSS.0000000000001045
|Authors||Trommelen, Jorn, Holwerda, Andrew M., Kouw, Imre W. K., Langer, Henning, Halson, Shona L., Verdijk, Lex B. and van Loon, Luc J. C.|
Introduction: We have previously shown that protein ingestion before sleep increases overnight muscle protein synthesis rates. Whether prior exercise further augments the muscle protein synthetic response to presleep protein ingestion remains to be established.
Objective: This study aimed to assess whether resistance-type exercise performed in the evening increases the overnight muscle protein synthetic response to presleep protein ingestion.
Methods: Twenty-four healthy young men were randomly assigned to ingest 30 g intrinsically L-[1-13C]-phenylalanine and L-[1-13C]-leucine-labeled casein protein before going to sleep with (PRO + EX, n = 12) or without (PRO, n = 12) prior resistance-type exercise performed in the evening. Continuous intravenous L-[ring- 2 H5]-phenylalanine, L-[1-13C]-leucine, and L-[ring- 2 H2]-tyrosine infusions were applied. Blood and muscle tissue samples were collected to assess whole-body protein balance, myofibrillar protein synthesis rates, and overnight incorporation of dietary protein-derived amino acids into de novo myofibrillar protein. Results: A total of 57% T 1% of the ingested protein-derived phenylalanine appeared in the circulation during overnight sleep. Overnight myofibrillar protein synthesis rates were 37% (0.055%Ihj1 T 0.002%Ihj1 vs. 0.040%Ihj1 T 0.003%Ihj1 , P G 0.001, based on L-[ring-2 H5]-phenylalanine) and 31% (0.073%Ihj1 T 0.004%Ihj1 vs. 0.055%Ihj1 T 0.006%Ihj1 , P = 0.024, based on L-[1-13C]-leucine) higher in PRO + EX compared with PRO. Substantially more of the dietary protein-derived amino acids were incorporated into de novo myofibrillar protein during overnight sleep in PRO + EX compared with PRO (0.026 T 0.003 vs. 0.015 T 0.003 molar percent excess, P = 0.012).
Conclusions: Resistance-type exercise performed in the evening augments the overnight muscle protein synthetic response to presleep protein ingestion and allows more of the ingested protein-derived amino acids to be used for de novo myofibrillar protein synthesis during overnight sleep.
|Keywords||sleep; recovery; exercise; muscle; stable isotopes; casein|
|Journal||Medicine and Science in Sports and Exercise|
|Journal citation||48 (12), pp. 2517-2525|
|Publisher||Lippincott Williams and Wilkins|
|Digital Object Identifier (DOI)||https://doi.org/10.1249/MSS.0000000000001045|
|Open access||Published as green open access|
|Research or scholarly||Research|
|Research Group||Mary MacKillop Institute for Health Research|
|Author's accepted manuscript|
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|Place of publication||United States of America|
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